Please use this identifier to cite or link to this item: http://imsear.hellis.org/handle/123456789/62912
Title: Differential acetylcholinesterase activity in rat cerebrum, cerebellum and hypothalamus.
Authors: Roy, Rini
Chaudhuri, Aditi Nag
Issue Date: 20-May-2006
Citation: Roy R, Chaudhuri AN. Differential acetylcholinesterase activity in rat cerebrum, cerebellum and hypothalamus. Indian Journal of Experimental Biology. 2006 May; 44(5): 381-6
Abstract: Acetylcholinesterase (AChE) has been purified from three different regions of rat brain using Sephadex G 200 column. SDS PAGE (6%) showed single band for the purified AChE fractions. Purified and lyophilized AChE from different (NH4)2SO4 precipitated fractions of three brain parts were utilized for in vitro enzyme kinetics using Dimethoate (Dmt) as inhibitor. K(m) values for cerebellum and hypothalamus were almost similar whereas cerebrum showed a different K(m) value compared to other two regions. With the drug Rivastigmine it was found that % G1 and G4 forms of AChE in three different parts of brain are different.
URI: http://imsear.hellis.org/handle/123456789/62912
Appears in Collections:Indian Journal of Experimental Biology

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